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Related Experiment Videos

Animal models in micromanipulation.

S Suzuki1, S Kurasawa, S Ohmura

  • 1Department of Obstetrics and Gynaecology, Keio University School of Medicine, Tokyo, Japan.

Annals of the Academy of Medicine, Singapore
|July 1, 1992
PubMed
Summary
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Cyclic adenosine monophosphate (cAMP) is crucial for maintaining oocyte meiotic arrest. Its decrease triggers oocyte maturation, while cytoplasmic factors are essential for early embryonic development after fertilization.

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Micromanipulation is a key technique in reproductive biology research.
  • Understanding the regulation of oocyte maturation and early embryonic development is critical.

Purpose of the Study:

  • To investigate the role of cyclic adenosine monophosphate (cAMP) in regulating mouse oocyte maturation.
  • To determine the activity of maturation promoting factor (MPF) in mouse eggs.
  • To explore the role of inositol trisphosphate (IP3) in sperm-induced egg activation.
  • To identify cytoplasmic factors influencing early mouse embryo development.

Main Methods:

  • Microinjection of anti-cAMP serum into oocyte cytoplasm.
  • Microinjection of cytoplasm from mature eggs into immature oocytes.

Related Experiment Videos

  • Detection of MPF activity in metaphase II oocytes.
  • Microinjection of varying concentrations of IP3 into mouse eggs.
  • Microinjection of morula or 2-cell embryo cytoplasm into 1-cell embryos.

    • Main Results:

    • Microinjection of anti-cAMP serum induced immature oocytes to resume meiosis, indicating cAMP's role in meiotic arrest.
    • MPF activity was detected in the cytoplasm of metaphase II oocytes.
    • IP3 microinjection caused a concentration-dependent conversion of zona glycoprotein ZP2 to ZP2f, mimicking fertilization.
    • Microinjection of early embryo cytoplasm released the 2-cell block in ICR mouse embryos, promoting development to the 4-cell stage.

    Conclusions:

    • Oocyte cAMP levels are essential for maintaining meiotic arrest; a decrease leads to maturation.
    • MPF is present and active in mature mouse oocytes.
    • IP3 plays a role in modifying the zona pellucida during egg activation.
    • Cytoplasmic factors are necessary for successful early embryonic development post-fertilization.