Total and free amino acids in the "Crinofizin" pineal extract

In order to establish the chemical nature of Crinofizin, a protidic extract obtained from bovine pineal, the amino acids content was analysed using two analytic programs: one for biologic fluids, the other for protein hydrolysates. The results showed that the pineal extract contains besides the common protein amino acids reported earlier, others, rare in proteins, such as hydroxyproline, hydroxylysine and 1-methylhistidine as well as non-protein amino acids. From among the latter, the alpha-aminoadipic acid, beta- alanine, O-cysteine, are found only in a bound form, and are detected only by hydrolysis. Other amino acids, such as taurine, glutamine, citruline are present only in free form whereas the alpha-amino-n-butyric acid, GABA, ethanolamine and ornithine are present in both forms, free or bound. The paper discusses the physiologic significance of some amino acids.