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Related Experiment Videos

[Non-cholera vibrios in enterobacteriologic routine].

V Magalhães1, M Magalhães, R A Lima

  • 1Departamento de Medicina Tropical, CCS/UFPE. Recife, PE, Brasil.

Revista Do Instituto De Medicina Tropical De Sao Paulo
|March 1, 1992
PubMed
Summary

A study in Brazil found Vibrio bacteria in only 1.7% of diarrheal stools, questioning the routine use of TCBS agar for diagnosing bacterial gastroenteritis.

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Area of Science:

  • Clinical microbiology
  • Bacteriology

Context:

  • Diarrheal diseases pose a significant public health challenge globally.
  • Accurate and efficient laboratory diagnosis is crucial for effective patient management and public health surveillance.
  • The identification of bacterial pathogens in stool samples aids in understanding disease etiology and epidemiology.

Purpose:

  • To investigate the prevalence of Vibrio species in diarrheal stools submitted for routine microbiologic diagnosis in Recife, Brazil.
  • To evaluate the utility and cost-effectiveness of using thiosulfate-citrate-bile salts-sucrose (TCBS) agar for Vibrio isolation in a clinical laboratory setting.

Summary:

  • Over a two-year period (May 1989-May 1991), 3250 diarrheal stool samples were analyzed.
  • Vibrio species were isolated from 55 samples (1.7% prevalence).

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  • The most common Vibrio species identified were V. parahaemolyticus (24 strains), V. furnissii (15 strains), and V. cholerae non-O1 (6 strains).

    • Impact:

    • The low isolation rate of Vibrio species suggests that routine use of TCBS agar may not be cost-effective in this specific clinical laboratory context.
    • Findings may inform laboratory diagnostic strategies and resource allocation for enteropathogen detection.
    • Highlights the importance of evaluating diagnostic methods for their clinical utility and economic feasibility.