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Related Experiment Videos

Preliminary results towards ciguatoxin immunodetection.

S Pauillac1, H Labrousse, A M Legrand

  • 1Institut Pasteur de Paris, Unité d'Immunocytochimie, France.

Bulletin De La Societe De Pathologie Exotique (1990)
|January 1, 1992
PubMed
Summary

Researchers developed an enzyme immunoassay using monensin as a model to detect ciguatoxin (CTX). High-affinity antibodies were produced, achieving a detection limit of 75 pg for monensin.

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Polyclonal and monoclonal antibodies to PbTx-2-type brevetoxins using minute amount of hapten-protein conjugates obtained in a reversed micellar medium.

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Area of Science:

  • Marine biology
  • Immunology
  • Analytical chemistry

Background:

  • Ciguatoxin (CTX) is a potent marine toxin responsible for ciguatera fish poisoning.
  • Purified CTX is scarce, hindering the development of sensitive detection methods.
  • Monensin, a polyether ionophore, shares structural similarities with CTX, making it a suitable model compound.

Purpose of the Study:

  • To develop a reliable enzyme immunoassay (EIA) for detecting CTX.
  • To produce specific antibodies against monensin as a surrogate for CTX detection.
  • To establish a sensitive detection limit for the developed immunoassay.

Main Methods:

  • Production of monensin-protein immunogen in bulk quantities.
  • Generation of rabbit polyclonal and mouse monoclonal antibodies (MAbs) against monensin.

Related Experiment Videos

  • Competitive micro-enzyme-linked immunosorbent assay (micro-ELISA) using MAb 2H8 in Terasaki plates.

    • Main Results:

    • High specificity and affinity antibodies against monensin were successfully produced.
    • The developed competitive micro-ELISA demonstrated a detection limit of 75 pg for free monensin.
    • No cross-reactivity was observed with CTX, indicating assay specificity.

    Conclusions:

    • Monensin serves as an effective model for developing CTX detection assays.
    • The generated monoclonal antibodies show high potential for sensitive CTX detection.
    • Further investigation is underway to adapt the assay for brevetoxin detection.