Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eclipse of the identifiable myoblast.

F E Stockdale1

  • 1Stanford University School of Medicine, California 95304-5306.

Symposia of the Society for Experimental Biology
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Patterning of fast and slow fibers within embryonic muscles is established independently of signals from the surrounding mesenchyme.

Development (Cambridge, England)·2001
Same author

Irx4 forms an inhibitory complex with the vitamin D and retinoic X receptors to regulate cardiac chamber-specific slow MyHC3 expression.

The Journal of biological chemistry·2001
Same author

Locally advanced breast cancer: is surgery necessary?

The breast journal·2001
Same author

Paclitaxel-induced hypersensitivity pneumonitis: radiographic and CT findings.

AJR. American journal of roentgenology·2001
Same author

Molecular and cellular biology of avian somite development.

Developmental dynamics : an official publication of the American Association of Anatomists·2000
Same author

Sonic hedgehog enhances somite cell viability and formation of primary slow muscle fibers in avian segmented mesoderm.

Anatomy and embryology·1999

Limb muscle development involves migrating cells from somites. A 34-hour gap exists where these myogenic cells are hard to identify, obscuring when cell fate is determined.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Muscle Development

Background:

  • Limb musculature originates from somite-derived cells that migrate into the limb bud.
  • A critical developmental window shows a ~34-hour period where myogenic cells are difficult to identify or culture.
  • Distinguishing migrating myogenic cells from non-migrating ones is challenging due to a lack of specific markers.

Purpose of the Study:

  • To investigate the developmental timing of myogenic cell identification and commitment during limb development.
  • To understand the cellular origins and migration dynamics of limb muscle precursor cells.
  • To clarify whether myogenic cell fate is determined during somite formation or post-migration.

Main Methods:

  • Analysis of myogenic cell populations in somites and limb buds during embryonic development.

Related Experiment Videos

  • Cell culture techniques to assess myogenic cell viability and differentiation potential.
  • Investigating potential markers for distinguishing migrating versus resident myogenic cell populations.

    • Main Results:

    • Myogenic cells are identifiable in somites before migration.
    • A significant hiatus exists in the detectability and culturability of myogenic cells migrating to the limb bud.
    • Current methods lack markers to differentiate between myogenic cells destined for axial versus peripheral muscles.

    Conclusions:

    • The precise timing of myogenic cell fate commitment remains unclear.
    • Further research is needed to identify specific markers for migrating myogenic cells.
    • Understanding this developmental window is crucial for comprehending limb muscle formation.