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The gastric H3 receptor: a review
The Yale Journal of Biology and Medicine
|November 1, 1992
Summary
Researchers identified the histamine H3 receptor (H3R) in human gastric cells, demonstrating its role in regulating gastric acid secretion. This discovery offers a molecular explanation for H3R
Area of Science:
- Pharmacology
- Gastroenterology
- Molecular Biology
Background:
- Previous studies suggested histamine H3 receptor involvement in gastric acid control.
- The H3 receptor's presence and function in non-neural cells, like gastric cells, remained largely uncharacterized.
Purpose of the Study:
- To investigate the presence and function of the histamine H3 receptor in human gastric tumoral cells (HGT-1).
- To biochemically characterize the H3 receptor in this non-neural cell line.
Main Methods:
- Pharmacological studies on HGT-1 cells.
- Solubilization and affinity purification of the H3 receptor.
- Biochemical characterization of the purified protein.
Main Results:
- HGT-1 cells express functional histamine H3 receptors.
- These receptors inhibit basal and carbachol-stimulated inositol phosphate formation.
- The H3 receptor was solubilized and purified as a 70 kDa protein.
Conclusions:
- This study provides the first biochemical description of the H3 receptor subtype.
- It is the first direct evidence of H3 receptor expression on a non-neural cell (gastric cell).
- These findings offer a molecular basis for the H3 receptor's inhibitory role in gastric physiology.