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Updated: Aug 18, 2026

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
Published on: March 5, 2018
[Effects of nitrogen nutritional deficiency on the distribution of particular lipids in a eutrophic lake milieu]
1Laboratoire de Zoologie et Protistologie U.A.-C.N.R.S. n.138, Université Blasie Pascal, Aubière, France.
Abstract:
The seasonal distribution of particulate lipids coupled with N-nutrient availability was studied in eutrophic Lake Aydat (Massif Central, France). The concentrations of lipids ranged between 196.9 and 2971.5 micrograms.l-1 (mean +/- s.d. = 1090.1 +/- 705.5 micrograms.l-1. Lipids were abundant in summer and fall when nitrates were insufficient reflecting thus an orientation of cell metabolism towards an accumulation of storage products. In such conditions, the heterocystous Cyanobacteria were found to develop due to their competitive advantage of exploiting atmospheric nitrogen. Their lipid metabolism did not seem to be affected at least partially by NO3- exhaustion.
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