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Human transfer factor: fractionation and biologic activity.

D R Burger, A A Vandenbark, D Daves

    Journal of Immunology (Baltimore, Md. : 1950)
    |September 1, 1976
    PubMed
    Summary
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    Human transfer factor (TF) fractions were analyzed for immune activity. Fraction III specifically transferred antigen reactivity in vivo, while other fractions modulated immune responses in vitro.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Human transfer factor (TF) is known to modulate immune responses.
    • Fractionation of TF is necessary to understand its diverse biological activities.

    Purpose of the Study:

    • To fractionate human transfer factor (TF) and characterize the biological activities of its components.
    • To determine the specific roles of different TF fractions in immune modulation in vivo and in vitro.

    Main Methods:

    • Exclusion chromatography was used to fractionate human TF.
    • Biological activity was assessed through in vivo assays (transfer of reactivity) and in vitro assays (mitogen activation, lymphocyte transformation).

    Main Results:

    • Specific in vivo immune transfer activity resided in Fraction III.

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  • Fraction I exhibited mitogenic and suppressive activities, while Fraction III augmented PHA and PWM responses and antigen-dependent lymphocyte transformation.
  • Fraction IV demonstrated suppressive effects on antigen-induced lymphocyte transformation.

    • Conclusions:

    • Human TF preparations contain distinct components responsible for both specific and non-specific immune modulation.
    • Fractionation reveals the complexity of TF, with different fractions mediating varied immune effects.