Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intrathecally injected antibody can diffuse into spinal cord.

Q P Ma1, Y S Shi, J S Han

  • 1Department of Physiology, Beijing Medical University, China.

The International Journal of Neuroscience
|July 1, 1992
PubMed
Summary

Intrathecal antibody injections can reach the spinal cord. This study shows anti-enkephalin serum diffuses into the spinal cord within 60 minutes, aiding neuropeptide research.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Short-term efficacy of mid-urethral sling with autologous fascia lata sling in the treatment of stress urinary incontinence].

Zhonghua fu chan ke za zhi·2025
Same author

[Expert consensus on diagnosis and treatment of chronic pelvic pain].

Zhonghua fu chan ke za zhi·2024
Same author

[Analysis of risk factors associated with postoperative pancreatic fistula after robotic-assisted distal pancreatectomy].

Zhonghua wai ke za zhi [Chinese journal of surgery]·2024
Same author

[Analysis of the common respiratory viruses in children with acute respiratory infection in a hospital in Lanzhou City from 2021 to 2022].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2023
Same author

Clinical predictors of polyps recurring in patients with chronic rhinosinusitis and nasal polyps: a systematic review and meta-analysis.

Rhinology·2023
Same author

[Long-term outcome of EVAHEART I implantable ventricular assist device for the treatment of end stage heart failure: clinical 3-year follow-up results of 15 cases].

Zhonghua xin xue guan bing za zhi·2023

Area of Science:

  • Neuroscience
  • Immunohistochemistry
  • Spinal Cord Research

Background:

  • Antibody microinjection is common for studying neuropeptide function.
  • Antibody diffusion in brain tissue remains poorly understood.

Purpose of the Study:

  • To investigate the diffusion capability of intrathecally injected antibodies into the spinal cord.
  • To determine the time course of antibody diffusion in the spinal cord.

Main Methods:

  • Immunohistochemical analysis of anti-enkephalin serum injected intrathecally.
  • Observation of enkephalin immunoreactivity and nonspecific staining in spinal cord tissue.

Main Results:

  • Enkephalin immunoreactivity appeared in spinal cord laminae I and II within 10-60 minutes post-injection.
  • Diffusion levels increased with time, from slight at 10 minutes to very clear at 60 minutes.
  • Nonspecific staining was noted in the dorsal white matter.

Conclusions:

  • Intrathecal antibodies can diffuse into the spinal cord within 10-60 minutes.
  • This diffusion supports the use of intrathecal antibody microinjection for neuropeptide function studies.
  • Understanding diffusion kinetics is crucial for interpreting antibody-based neuropharmacological experiments.

Related Experiment Videos