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[RC-IAL: rabbit kidney continuous cell line--characteristics and substrate for viral replication]
A S Cruz1, C A Figueiredo, M L Barbosa
1Instituto Adolfo Lutz, São Paulo, Brasil.
Revista De Saude Publica
|December 1, 1992
Summary
The rabbit kidney cell line RC-IAL maintains stable characteristics, including fibroblast morphology and consistent cloning efficiency. This cell line is confirmed to be free of contaminants, making it suitable for public health research.
Area of Science:
- Cell Biology
- Virology
- Animal Cell Culture
Context:
- Characterization of established cell lines is crucial for reliable scientific research.
- The RC-IAL cell line, derived from rabbit kidney cells, has been maintained for extensive passage levels.
Purpose:
- To comprehensively analyze and document the biological characteristics of the RC-IAL rabbit kidney cell line.
- To confirm the species of origin and assess viral susceptibility for potential research applications.
Summary:
- The RC-IAL cell line exhibits stable fibroblast-like morphology and consistent cellular growth with a cloning efficiency of approximately 9%.
- Chromosomal analysis indicates modal numbers consistent with the rabbit species, with minor variations.
- The cell line's origin was verified, and its susceptibility to vaccinia, cowpox, herpes simplex (types 1 and 2), and rubella viruses was confirmed, demonstrating cytopathic effects.
Impact:
- The characterized RC-IAL cell line serves as a reliable and contaminant-free substrate for scientific investigations.
- Its confirmed viral susceptibility and stability enhance its value for public health research and virology studies.