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Related Experiment Videos

Lineage switch macrophages can present antigen.

J D Bretz1, S C Chen, D Redenius

  • 1Department of Microbiology, Michigan State University, East Lansing 48824-1101.

Developmental Immunology
|January 1, 1992
PubMed
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Newly identified "lineage switch" cells, derived from pre-B cells, function as effective antigen-presenting cells, demonstrating macrophage capabilities. This challenges traditional views on hematopoietic lineage plasticity.

Area of Science:

  • Immunology
  • Cell Biology
  • Hematopoiesis

Background:

  • Reports of lymphoid-to-macrophage lineage switching raise questions about the functionality of these cells.
  • It remains unclear if these

Purpose of the Study:

  • To determine if "lineage switch" cells derived from pre-B cells exhibit functional macrophage characteristics, specifically antigen presentation.
  • To investigate the plasticity of hematopoietic lineage commitment.

Main Methods:

  • Utilized a T-cell hybridoma assay to assess antigen-presenting capacity.
  • Co-cultured "lineage switch" cell lines with chicken ovalbumin (cOVA) and a cOVA-specific T-cell hybridoma.
  • Compared antigen presentation by "lineage switch" cells with original pre-B cells, B-cell lymphomas, and peritoneal exudates.

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Main Results:

  • "Lineage switch" cell lines effectively presented antigen in a Major Histocompatibility Complex (MHC)-restricted manner, similar to macrophages.
  • Original pre-B cells and derived B-cell lymphomas did not present antigen.
  • These cells rearranged their kappa light-chain immunoglobulin locus, indicating co-expression of lymphoid and myeloid markers.

Conclusions:

  • "Lineage switch" cells are functional macrophages capable of antigen presentation.
  • Macrophage differentiation and immunoglobulin rearrangement are not mutually exclusive.
  • Hematopoietic lineage commitment exhibits greater plasticity than previously understood.