Mixed function oxidase demethylase and dealkylase activity in an electromagnetic field

In contrast to the increase in reaction rate of microsomal NADPH-cytochrome-P450 reductase activity resulting from low-level microwave perturbation (reported earlier) transformations involving the entire MFO-pathway were inhibited by a microwave field. Dealkylation of 7-ethoxycoumarin was inhibited 25% and demethylation of p-nitroanisole was inhibited 40% when the reaction was carried forward in a 9.14 GHz CW field. Microsomal preparations from the liver of mature chickens had enzymic characteristics (kinetic constants, inhibitor-response spectrum) for these substrates similar to those reported for rodent and human MFO complex.