Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The pathogenesis of hemophilia; an experimental analysis of the anticephalin hypothesis in hemophilic dogs.

J B GRAHAM, E M BARROW

    The Journal of Experimental Medicine
    |August 1, 1957
    PubMed
    Summary

    Experiments in hemophilic dogs challenge the "anticephalin" hypothesis for hemophilia. Findings suggest the biphasic dilution curve is an artifact, not evidence of an inhibitor, and that canine hemophilia pathogenesis doesn't require an "anticephalin".

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Respiratory, Blood, and Heart Enzymatic Adaptations of Sebastolobus alascanus (Scorpaenidae; Teleostei) to the Oxygen Minimum Zone: A Comparative Study.

    The Biological bulletin·2018
    Same author

    How It Was: Pathology at UNC 1896-1973.

    Haemophilia : the official journal of the World Federation of Hemophilia·2016
    Same author

    Elasmobranch pericardial function. 1. Pericardial pressures are not always negative.

    Fish physiology and biochemistry·2013
    Same author

    Elasmobranch pericardial function 2. The influence of pericardial pressure on cardiac stroke volume in horn sharks and blue sharks.

    Fish physiology and biochemistry·2013
    Same author

    Development of an MHC class I L(d)-restricted PSA peptide-loaded tetramer for detection of PSA-specific CD8+ T cells in the mouse.

    Prostate cancer and prostatic diseases·2011
    Same author

    Effects of expected global climate change on marine faunas.

    Trends in ecology & evolution·2011

    Area of Science:

    • Hematology
    • Hemostasis
    • Canine Research

    Background:

    • The "anticephalin" hypothesis proposes an inhibitor in hemophilia pathogenesis.
    • Previous studies in humans suggested this hypothesis based on specific experimental observations.
    • Replicating these experiments in hemophilic dogs allows for extended investigation.

    Purpose of the Study:

    • To investigate the validity of the "anticephalin" hypothesis in canine hemophilia.
    • To re-evaluate key experiments suggesting an "anticephalin" using hemophilic dog models.
    • To determine if alternative explanations exist for observed phenomena in hemophilia.

    Main Methods:

    • Contact activation with asbestos on hemophilic dog plasma.
    • Analysis of plasma ionic strength and dilution effects.
    Keywords:
    HEMOPHILIA/etiology and pathogenesis

    Related Experiment Videos

  • Assessment of prothrombin utilization in hemophilic dog blood.
  • Ether extraction of plasma components and subsequent transfusion studies.

    • Main Results:

    • Asbestos activation shortened clotting time but did not correct defective prothrombin utilization.
    • The biphasic dilution curve was demonstrated to be an artifact of uncontrolled ionic strength.
    • Ether extraction removed fibrinogen and reduced prothrombin; generated clotting activity was not anti-hemophilic factor (AHF).
    • Transfusions of treated plasma did not improve prothrombin utilization in hemophilic dogs.

    Conclusions:

    • The four key experiments supporting the "anticephalin" hypothesis are reproducible in dogs.
    • These phenomena are explainable by factors other than an "anticephalin", rendering the hypothesis unnecessary for canine hemophilia.
    • The findings suggest the "anticephalin" hypothesis is also not applicable to human hemophilia.