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Canine distemper virus transcripts sequenced from pagetic bone.

M T Gordon1, A P Mee, D C Anderson

  • 1University of Manchester, Dept. of Cell and Structural Biology, Manchester, UK.

Bone and Mineral
|November 1, 1992
PubMed
Summary
This summary is machine-generated.

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Canine distemper virus and other paramyxoviruses are found in bone cells of Paget's disease patients. Viral mutations may explain the persistent nature of this chronic skeletal disease.

Area of Science:

  • Virology
  • Skeletal Biology
  • Genetics

Background:

  • Paget's disease is a chronic skeletal disorder with an unknown viral etiology.
  • Paramyxoviruses are suspected causative agents, with previous studies implicating distemper virus.

Purpose of the Study:

  • To confirm the presence of distemper virus and identify other paramyxoviruses in Paget's disease bone cells.
  • To investigate potential viral mutations contributing to disease persistence.

Main Methods:

  • Reverse transcription of RNA from pagetic bone.
  • Polymerase chain reaction (PCR) amplification for distemper and measles sequences.
  • Dideoxy sequencing of PCR products.

Main Results:

Related Experiment Videos

  • Distemper virus nucleic acids detected in 8/13 patients; measles virus in 1/10 patients.
  • One patient showed evidence of both viruses.
  • Sequencing revealed 2% base pair changes in distemper virus sequences compared to a reference strain.

    • Conclusions:

    • Canine distemper and potentially other paramyxoviruses reside within bone cells in Paget's disease.
    • Mutations in the viral genome may contribute to the chronic and persistent nature of the disease.