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Related Experiment Videos

Keratinocyte activation following T-lymphocyte binding.

T J Stoof1, R S Mitra, V Sarma

  • 1Department of Pathology, University of Michigan Medical Center, Ann Arbor.

The Journal of Investigative Dermatology
|January 1, 1992
PubMed
Summary

Direct T cell binding to keratinocytes activates skin cells, releasing inflammatory signals. This adhesion-mediated process amplifies cutaneous inflammation and influences immune cell entry into the skin.

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Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • T lymphocytes are known to infiltrate the skin during inflammation.
  • The precise mechanisms by which T cells interact with and activate skin cells, like keratinocytes, are not fully understood.

Purpose of the Study:

  • To investigate whether direct physical binding between T lymphocytes and keratinocytes triggers activation pathways in keratinocytes.
  • To elucidate the role of cell-to-cell adhesion in initiating inflammatory responses within the skin.

Main Methods:

  • Co-culture of CD3+ T cells (HUT 78) with non-cytokine-treated cultured keratinocytes, allowing direct physical binding.
  • Analysis of mRNA expression in keratinocytes for specific inflammatory mediators (TNF-alpha, IL-6, ICAM-1) following T cell adhesion.

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  • Comparison with control cultures where T cells were not in direct contact with keratinocytes.

    • Main Results:

    • Direct physical binding of T cells to keratinocytes induced the mRNA expression of tumor necrosis factor-alpha, interleukin-6, and intercellular adhesion molecule-1.
    • This keratinocyte activation was mRNA-specific, with no significant changes observed in other tested mRNAs.
    • The activation was one-sided, as no changes in T cell mRNA levels were detected.
    • Activation was dependent on direct cell-to-cell contact, as separated co-cultures showed no effect.

    Conclusions:

    • Direct adhesion between T lymphocytes and keratinocytes is sufficient to activate keratinocytes.
    • This adhesion-mediated activation may play a crucial role in amplifying cutaneous inflammation.
    • The interaction contributes to the trafficking and localization of inflammatory cells within the skin.