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The presence of free D-alanine, D-proline and D-serine in mice
Y Nagata1, K Yamamoto, T Shimojo
1Department of Biochemistry, Sapporo Medical College, Hokkaido, Japan.
Biochimica Et Biophysica Acta
|January 23, 1992
Abstract:
The presence of free D-alanine, D-proline and D-serine was demonstrated in mammalian tissues, using a mutant mouse strain lacking D-amino acid oxidase. In the experiment, free amino acids from the kidney and serum were derivatized with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (FDAA) to diastereomers, separated by two-dimensional thin-layer chromatography (TLC), and analysed by reversed-phase high-performance liquid chromatography (HPLC) for the resolution of D- and L-isomers. D/L ratios of alanine, proline and serine were obtained based on the peak areas of HPLC.