Inactivation of placental factor XIIIa by acrylamide

Acrylamide rapidly and irreversibly inactivates thrombin activated Factor XIIIa, without affecting neither the intact zymogen nor its proteolytic activation. The inactivation is strictly dependent on the presence of calcium ions and is accompanied by a decrease in the number of free and total thiol residues, suggesting that cysteine residue(s), whose reactivity is modulated by calcium, is (are) probably responsible for the acrylamide-directed inactivation.