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Insertion/deletion polymorphism and other restriction fragment length polymorphisms in the MCC gene
Y Miyoshi1, I Nishisho, Y Miki
1Department of Biochemistry, Cancer Institute, Tokyo.
Japanese Journal of Cancer Research : Gann
|January 1, 1992
Summary
The MCC gene shows potential as a tumor suppressor for colorectal neoplasms. New DNA variations (polymorphisms) in the MCC gene can aid in diagnosing familial adenomatous polyposis (FAP) and tracking tumor development.
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- The MCC gene is a candidate tumor suppressor gene implicated in colorectal neoplasms.
- MCC is genetically linked to the familial adenomatous polyposis (FAP) locus.
Purpose of the Study:
- To identify and characterize polymorphic systems within the MCC gene.
- To assess the utility of these polymorphisms for presymptomatic diagnosis in FAP and for studying tumor development.
Main Methods:
- Identification of five polymorphic systems in MCC gene (cDNA and genomic).
- Characterization of an insertion/deletion polymorphism detectable by polymerase chain reaction (PCR).
Main Results:
- Five restriction fragment length polymorphism (RFLP) systems were identified in the MCC gene.
- One novel insertion/deletion polymorphism was found, detectable by PCR.
Conclusions:
- The identified MCC gene RFLP systems are valuable tools for linkage studies in FAP.
- These polymorphisms can be used to investigate loss of heterozygosity in colonic polyps and tumors, aiding in cancer research.