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Updated: Jul 6, 2026

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Transcription factor loading on the MMTV promoter: a bimodal mechanism for promoter activation
T K Archer1, P Lefebvre, R G Wolford
1Hormone Action and Oncogenesis Section, Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
Abstract:
The mouse mammary tumor virus (MMTV) promoter attains a phased array of six nucleosomes when introduced into rodent cells. This architecture excludes nuclear factor 1/CCAAT transcription factor (NF1/CTF) from the promoter before glucocorticoid treatment and hormone-dependent access of nucleolytic agents to promoter DNA. In contrast, when the promoter was transiently introduced into cells, NF1/CTF was bound constitutively and nucleolytic attack was hormone-independent. Thus, induction at this promoter was a bimodal process involving receptor-dependent remodeling of chromatin that allows NF1/CTF loading and direct receptor-mediated recruitment of additional transcription factors.
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