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Antioxidant effect on renal scarring following infection of mannose-sensitive-piliated bacteria
T Matsumoto1, Y Mitzunoe, N Ogata
1Department of Urology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Abstract:
Renal scars have been considered to occur in later stages of chronic pyelonephritis. In our experimental pyelonephritis model, bacteria which possessed mannose-sensitive (MS) pili on the surface promoted renal scarring following inoculation to the renal parenchyma. Polyethylene glycol-modified superoxide dismutase (PEG-SOD) and 2-O-octadecylascorbic acid (CV3611) significantly suppressed scarring when administered orally or parenterally during the early stage of kidney infection with MS-piliated bacteria. These findings suggest that the superoxide and other active oxygens play an important role in renal scarring following infection and that PEG-SOD and CV3611 may be agents capable of preventing renal scarring following bacterial pyelonephritis.
Insights
Mannose-sensitive (MS) pili promote renal scarring in experimental pyelonephritis. Treatments with polyethylene glycol-modified superoxide dismutase (PEG-SOD) and CV3611 suppressed scarring, suggesting their potential for preventing kidney damage from bacterial infections.
Area of Science:
- Nephrology
- Microbiology
- Pharmacology
Background:
- Renal scarring is a known complication of chronic pyelonephritis.
- The role of bacterial virulence factors, such as mannose-sensitive (MS) pili, in initiating renal scarring is not fully understood.
- Superoxide and reactive oxygen species are implicated in inflammatory processes within the kidney.
Purpose of the Study:
- To investigate the role of MS-piliated bacteria in promoting renal scarring in an experimental pyelonephritis model.
- To evaluate the efficacy of polyethylene glycol-modified superoxide dismutase (PEG-SOD) and 2-O-octadecylascorbic acid (CV3611) in preventing renal scarring.
- To elucidate the involvement of superoxide in the pathogenesis of renal scarring during bacterial kidney infections.
Main Methods:
- Establishment of an experimental pyelonephritis model using MS-piliated bacteria.
- Administration of PEG-SOD and CV3611 via oral or parenteral routes during the early stage of infection.
- Assessment of renal scarring following bacterial inoculation and treatment.
Main Results:
- Bacteria possessing MS pili significantly promoted renal scarring after inoculation into the renal parenchyma.
- Both PEG-SOD and CV3611 demonstrated significant suppression of renal scarring.
- Therapeutic effects were observed when treatments were administered during the early phase of kidney infection.
Conclusions:
- Superoxide and other active oxygen species play a critical role in the development of renal scarring following bacterial pyelonephritis.
- PEG-SOD and CV3611 show promise as therapeutic agents for preventing renal scarring associated with bacterial kidney infections.
- Targeting oxidative stress pathways may be a viable strategy for mitigating kidney damage in pyelonephritis.