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Coexpression of CD4 and CD8 on mitogen-activated peripheral blood T cells from children with asthma: possible

U Schauer1, I Kohl, R Jäger

  • 1Universitäts-Kinderklinik, Philipps Universität, Marburg, Federal Republic of Germany.

Annals of Allergy
|April 1, 1992
PubMed

Insights

Asthmatic children show higher CD8 T cell expression compared to healthy children. Interleukin 4 may preactivate lymphocytes in asthmatic patients, affecting T cell coexpression.

Area of Science:

  • Immunology
  • Pediatric Allergy

Background:

  • Asthma is a chronic inflammatory airway disease.
  • T cell activation and surface antigen expression are critical in asthma pathogenesis.

Purpose of the Study:

  • To investigate T cell surface antigen expression in children with asthma.
  • To explore the role of interleukin 4 in T cell coexpression in asthmatic children.

Main Methods:

  • Isolation of mononuclear cells from asthmatic and healthy children.
  • Phytohemagglutinin stimulation and MTT staining for cell activation.
  • Indirect immune fluorescence using CD4 and CD8 antibodies to detect antigen expression.

Main Results:

  • Enhanced expression of CD8 on mitogen-activated T cells in asthmatic children (43% vs 27%).
  • Significantly increased coexpression of CD4 and CD8 on activated lymphocytes from asthmatic patients.
  • Interleukin 4 enhanced CD4/CD8 coexpression in healthy but not asthmatic children's lymphocytes.

Conclusions:

  • Asthma is associated with altered T cell activation and CD8 expression.
  • Lymphocytes from asthmatic children may be preactivated in vivo by interleukin 4.
  • Findings suggest unique immune responses in pediatric asthma.

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