Effects of acid Ca2+ Ringer on passive electrical properties and intracellular ion activities in leech Retzius neuron

1. A significant drop in effective input resistance of the free membrane and an increase in effective coupling resistance in acid Ca2+ Ringer (complete replacement of Na+ with Ca2+, pH 4) compared to control medium has been obtained in leech Retzius neurons. 2. In neutral Ca2+ Ringer (pH 7.2), effective input resistance increased while effective coupling resistance did not change. In acid sodium, leech Ringer (pH 4) effective input resistance increased while coupling resistance decreased. 3. Ten millimolar manganese and 10 mmol tetraethylammonium did not block conductance changes obtained in acid Ca2+ Ringer. 4. Intracellular activity of Na+ decreased, cellular activity of Cl- increased and intracellular K+ activity was unchanged in both acid and neutral Ca2+ Ringer. 5. The main difference was intracellular acidification in acid Ca2+ Ringer while intracellular pH was unchanged in neutral Ca2+ Ringer. 6. We discuss the possibility that in acid Ca2+ Ringer, intracellular acidification in leech neurons may be responsible for accompanying conductive changes.