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The dynamics of histoenzymatic changes in the kidney in acute poisoning with benzene
Abstract:
The experiments were carried out in mice which were divided into 4 experimental groups and a control group. In the course of the experiment SD, NADH2-tetrazolium reductase; ATP-ase, G-6-P-ase and AP were observed. It was found that acute benzene intoxication causes the disturbances in the enzymatic activities of the cells of the main segment of the nephron. The impairment of tissue respiration and oxygen phosphorylation and of active transport is due to benzene intoxication. Benzene leads to injury of the endoplasmatic reticulum in the cells of the kidney.
Insights
Acute benzene exposure disrupts kidney function in mice by impairing cellular enzymes and damaging the endoplasmic reticulum. This study reveals benzene
Area of Science:
- Toxicology
- Biochemistry
- Cell Biology
Background:
- Benzene is a common industrial solvent with known toxic effects.
- Kidney damage is a potential consequence of benzene exposure.
- Understanding the specific cellular mechanisms of benzene toxicity is crucial.
Purpose of the Study:
- To investigate the impact of acute benzene intoxication on kidney enzymatic activities in mice.
- To elucidate the cellular basis of benzene-induced nephrotoxicity.
Main Methods:
- Mice were divided into four experimental groups and one control group.
- Observed enzymatic activities included succinate dehydrogenase (SD), NADH2-tetrazolium reductase, ATP-ase, glucose-6-phosphatase (G-6-P-ase), and alkaline phosphatase (AP).
- Kidney tissue was examined for cellular damage, specifically to the endoplasmic reticulum.
Main Results:
- Acute benzene intoxication significantly disturbed enzymatic activities in the main segment of the nephron.
- Benzene exposure impaired tissue respiration and oxygen phosphorylation.
- Active transport mechanisms within kidney cells were compromised.
- Benzene induced injury to the endoplasmic reticulum in kidney cells.
Conclusions:
- Acute benzene intoxication causes significant disruptions in kidney cellular functions.
- Impairment of energy metabolism and active transport are key mechanisms of benzene nephrotoxicity.
- Benzene directly damages the kidney's endoplasmic reticulum, contributing to its toxic effects.