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Restriction endonuclease analysis of Aspergillus fumigatus DNA
J P Burnie1, A Coke, R C Matthews
1Department of Medical Microbiology, University of Manchester Medical School.
Aims:
To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould.
Methods:
DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA was restricted by EcoRI and Xba I.
Results:
All isolates were identical when cut by EcoRI; Xba I delineated six DNA types.
Conclusions:
DNA fingerprinting can be used to type isolates of A fumigatus. Strains from within an aspergilloma which were morphologically distinct could either have the identical DNA fingerprint or produce a unique type.