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Fimbriae and membranes on Haemophilus parasuis
1Institut für Mikrobiologie und Tierhygiene, Elektronenmikroskopie des Fachbereiches Veterinärmedizin der Freien Universität Berlin, Germany.
Abstract:
In the electron microscope an additional layer (glycocalix) of the cell wall and fimbriae on Haemophilus parasuis were shown in thin sections of the infected CAM which have their origin on the CM of the Haemophilus parasuis-cells. No fimbriation was seen after conventional cultivation.
Insights
Electron microscopy revealed a glycocalyx and fimbriae on Haemophilus parasuis within infected CAM, originating from the cell membrane. Conventional cultivation methods did not show these structures.
Area of Science:
- Microbiology
- Cell Biology
- Microscopy
Background:
- Haemophilus parasuis is a significant pathogen in swine.
- Understanding bacterial surface structures is crucial for pathogenesis studies.
Purpose of the Study:
- To investigate the ultrastructure of Haemophilus parasuis in infected CAM using electron microscopy.
- To determine if fimbriae are present on Haemophilus parasuis during infection and compare with in vitro cultivation.
Main Methods:
- Transmission electron microscopy (TEM) of thin sections from infected Chorioallantoic Membrane (CAM).
- Conventional bacterial cultivation techniques.
Main Results:
- TEM revealed an additional layer, the glycocalyx, on the Haemophilus parasuis cell wall.
- Fimbriae originating from the cell membrane were observed on Haemophilus parasuis within infected CAM.
- No fimbriae were detected on Haemophilus parasuis after conventional cultivation.
Conclusions:
- The glycocalyx and fimbriae are present on Haemophilus parasuis during CAM infection.
- These surface structures may play a role in Haemophilus parasuis pathogenesis.
- Conventional cultivation may not accurately reflect the in vivo morphology of Haemophilus parasuis.