Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nickel contact sensitivity in the guinea pig. An efficient open application test method.

G D Nielsen1, A E Rohold, K E Andersen

  • 1Department of Environmental Medicine, Institute of Environmental Health, Odense University, Denmark.

Acta Dermato-Venereologica
|January 1, 1992
PubMed
Summary

This study demonstrates that nickel sulfate effectively induces contact sensitivity in guinea pigs. The developed nickel allergy was confirmed to be long-lasting in the animal model.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The epidemic of methylisothiazolinone contact allergy in Europe: follow-up on changing exposures.

Journal of the European Academy of Dermatology and Venereology : JEADV·2019
Same author

Sociodemographic factors do not have a large influence on adherence to topical treatment in patients with psoriasis.

The British journal of dermatology·2019
Same author

Use of topical antipsoriatic drugs in Denmark: a nationwide drug utilization study.

The British journal of dermatology·2018
Same author

A smartphone application supporting patients with psoriasis improves adherence to topical treatment: a randomized controlled trial.

The British journal of dermatology·2018
Same author

Insulation fiber deposition in the airways of men and rats. A review of experimental and computational studies.

Regulatory toxicology and pharmacology : RTP·2018
Same author

Comment on: "Human symptom responses to bioeffluents, short-chain carbonyl/acids and long-chain carbonyls in a simulated aircraft cabin environment" by Weisel et al., Indoor Air (2017).

Indoor air·2017

Area of Science:

  • Dermatology
  • Immunology
  • Toxicology

Background:

  • Nickel is a common contact allergen responsible for allergic contact dermatitis.
  • Developing reliable methods to induce and study nickel allergy is crucial for understanding sensitization mechanisms.

Purpose of the Study:

  • To establish a reproducible method for inducing nickel contact sensitivity in guinea pigs.
  • To evaluate the efficacy of different nickel sulfate concentrations and vehicles in sensitization.

Main Methods:

  • Guinea pigs were pretreated with sodium lauryl sulfate and then treated with nickel sulfate in lanolin cream or hydroxypropyl cellulose.
  • Intradermal injections of aluminium potassium sulfate were used as an adjuvant.
  • Animals were challenged with nickel sulfate, and sensitization rates were assessed.

Related Experiment Videos

Main Results:

  • Nickel sulfate concentrations of 1% and 3% in lanolin cream, and 1% in hydroxypropyl cellulose, significantly induced contact sensitivity.
  • Sensitization frequencies ranged from 57% to 100% depending on the concentration and vehicle used.
  • Rechallenge confirmed the development of a lasting nickel allergy.

Conclusions:

  • The open epicutaneous application method with nickel sulfate is effective in inducing contact sensitivity in guinea pigs.
  • Lanolin cream and hydroxypropyl cellulose are suitable vehicles for nickel sensitization.
  • This model provides a reliable platform for studying nickel allergy and testing potential interventions.