Accessibility and function of human thyroid epithelial cells in monolayer culture

Measurement of thyroid stimulating immunoglobulins (TSI) has not yet found widespread application in the diagnosis and management of Graves' disease. One of the problems is the poor and variable sensitivity of the different assays. We therefore studied the influence of the accessibility of the basal part of human thyroid epithelial cells in monolayer culture on their cAMP response to thyroid stimulating hormone (TSH) and TSI. Test media containing either ammoniumsulphate-precipitated globulin fractions of sera from normal controls and treated or untreated patients with Graves' disease or TSH were used to stimulate cAMP production by cryopreserved human thyroid epithelial cells in monolayer culture. Incubations with and without the use of porous membrane inserts as culture surface were compared by univariate parametric and non-parametric testing. It appears that more TSH-receptors, probably on the basal part of the thyrocyte, can be exposed to the medium by using a porous membrane as culture surface as demonstrated by the increased analytical sensitivity of the semi-bioassay of TSH and TSI.