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Assignment of the chicken tyrosine hydroxylase gene to chromosome 6 by FISH
M Dominguez-Steglich1, A Carrier, C Auffray
1Department of Human Genetics, University of Würzburg, FRG.
Cytogenetics and Cell Genetics
|January 1, 1992
Abstract:
The gene for tyrosine hydroxylase, the first and rate-limiting enzyme in the biosynthetic pathway of catecholamine neurotransmitters, has been localized in situ to chromosome 6 in the chicken. It is the first DNA marker to be reported for this telocentric macrochromosome. Use of a 45-kb biotinylated chicken-specific cosmid probe and a sensitive fluorescent detection system proved to be highly efficient, with over 90% of metaphases showing positive hybridization signals on one or (usually) both chromosome 6 homologs, in physically mapping this single-gene locus.