Effect of atrial natriuretic peptide on apomorphine-induced stereotyped cage-climbing behavior in mice

In previous experiments, it was observed that rat atrial natriuretic peptide-(1-28) (ANP-(1-28)) participated in fear-induced learning and memory processes via dopaminergic and cholinergic mediation. Since cage-climbing behavior is described as a simple test for studying dopaminergic activity in the central nervous system, a systemic study was carried out with ANP-(1-28) in order to confirm or to exclude the possible involvement of dopamine in the ANP-induced action in the brain. The present study demonstrate that ANP-(1-28) facilitated cage-climbing behavior in mice in a dose-dependent manner. When combined with apomorphine, the peptide potentiated the effect of the dopamine agonist. The effect of ANP-(1-28) in combination with apomorphine could be antagonized by a selected dose of haloperidol. These data suggest that ANP might be regarded as a dopamine agonist-modulating agent and that a dopaminergic mechanism is a possible mode of action of ANP in the fear-induced learning studied earlier.