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Murine Hox-1.11 homeobox gene structure and expression
D P Tan1, J Ferrante, A Nazarali
1Laboratory of Biochemical Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892.
Summary
The Hox-1.11 gene, crucial for embryonic development, is located on mouse chromosome 6. Its expression is highest in 12-day-old embryos, particularly in the hindbrain and developing organs.
Area of Science:
- Developmental Biology
- Genetics
- Molecular Biology
Background:
- The Hox-1.11 gene encodes a protein with a homeodomain, a conserved pentapeptide, and an acidic region.
- Its homeodomain sequence is identical to Hox-2.8, and its N- and C-terminal regions resemble human HOX2H.
Purpose of the Study:
- To characterize the Hox-1.11 gene and its expression patterns during mouse embryonic development.
Main Methods:
- Gene mapping to identify the chromosomal location of Hox-1.11.
- RNA detection using poly(A)+ RNA analysis.
- In situ hybridization to determine precise expression sites.
Main Results:
- Hox-1.11 gene is located on murine chromosome 6 within the Hox-1 cluster.
- A 1.7 kb poly(A)+ RNA species of Hox-1.11 was detected in mouse embryos.
- Expression is most abundant at 12 days of embryonic development and decreases thereafter.
- Anterior expression is observed in the mid and posterior hindbrain, cranial ganglia VII and VIII, spinal cord, and various developing organs.
Conclusions:
- Hox-1.11 plays a role in the development of the hindbrain and other embryonic structures.
- Its temporal and spatial expression patterns provide insights into its function during embryogenesis.