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Related Experiment Videos

Carboxyl-terminal deletion and point mutations decrease the transforming potential of the activated rat neu oncogene

Y Mikami1, J G Davis, K Dobashi

  • 1Division of Immunology, University of Pennsylvania School of Medicine, Philadelphia 19104-6082.

Proceedings of the National Academy of Sciences of the United States of America
|August 15, 1992
PubMed
Summary
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The carboxyl-terminal domain of the rat neu protein (p185Tneu) is crucial for its cell growth-regulating properties. Mutations affecting this region, including autophosphorylation sites, reduce the neu protein

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Oncogenesis

Background:

  • The rat neu oncogene encodes p185Tneu, a growth factor receptor/transmembrane tyrosine kinase.
  • p185Tneu is structurally similar to the epidermal growth factor receptor but distinct.
  • Its activity regulation by the carboxyl-terminal region and autophosphorylation sites is not fully understood.

Purpose of the Study:

  • To investigate the role of the carboxyl-terminal region and putative autophosphorylation sites in regulating p185Tneu activity.
  • To determine how these regions influence the transforming capacity of p185Tneu.

Main Methods:

  • Site-directed mutagenesis was used to create p185Tneu mutants.
  • Mutants included replacement of a tyrosine autophosphorylation site (residue 1253) with phenylalanine and deletion of the carboxyl-terminal 122 amino acids.

Related Experiment Videos

  • Proteins were expressed in NIH 3T3 cells and analyzed for autophosphorylation, substrate phosphorylation, oligomerization, and transforming capacity.

    • Main Results:

    • Mutant p185Tneu proteins showed comparable autophosphorylation, substrate phosphorylation, oligomerization, and responsiveness to neu-activating factor.
    • However, these mutants exhibited significantly decreased in vitro and in vivo transforming capacity.
    • The carboxyl-terminal domain and at least one autophosphorylation site positively regulate neu protein's cell growth-regulating properties.

    Conclusions:

    • The carboxyl-terminal domain of p185Tneu is essential for its full transforming activity.
    • Specific tyrosine autophosphorylation sites within this domain are critical for regulating cell growth.
    • These findings highlight the importance of the carboxyl-terminal region in neu-mediated oncogenesis.