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Some technical parameters influencing the precision and accuracy of fragment size determination for RFLP's
C Puers1, P Wiegand, B Brinkmann
1Institut für Rechtsmedizin, Universität Münster, Bundesrepublik Deutschland.
International Journal of Legal Medicine
|January 1, 1992
Abstract:
A comparison was carried out between 3 computer-assisted systems for the estimation of DNA fragment length: (1) the "Digitab" system (developed at our institute), (2) the FBI analysis system (FBI Quantico, USA) and (3) the BioImage system (Waters/Millipore, USA). Both the FBI system and the BioImage system were found to be much more precise than the manual Digitab system. In an additional experiment, a possible influence of the field strength on the "accuracy" of fragment size measurement was checked. Lowering the field strength to approx. 1V/cm led to a statistically significant increase in the measured fragment size.