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Updated: Oct 14, 2026

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
Adrenergic receptor-mediated increase of intracellular Ca2+ concentration in isolated bovine corneal epithelial cells
P S Reinach1, R R Socci, C Keith
1Department of Physiology and Endocrinology, Medical College of Georgia, Augusta 30912-3000.
Abstract:
1. We determined if Ca2+ is a second messenger for adrenergic receptor-effector coupling in bovine corneal epithelial cells. 2. Methoxamine (10(-5) M) selectively increased intracellular Ca2+ concentration (Cai) by 65%. This increase was only partially suppressed through the removal of extracellular Ca2+ or pretreatment with 10(-6) M verapamil. 3. The beta-adrenergic-mediated increases in Cai were entirely dependent on extracellular Ca2+. These increases were directly elicited through stimulation of adenylate cyclase because 10(-6) M isoproterenol and the active analogues of forskolin (10(-5) M) all elevated Cai. 4. Therefore, increases in Cai serve a second messenger function for alpha-1 and beta-adrenergic receptor-effector coupling.
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