[Leukocyte migration inhibition test in skin chamber--an in-vivo method]

For the demonstration of a cell-mediated sensitisation, tuberculin allergics, chromate allergics, penicillin allergics, and controls were investigated. The method used was the measurement of leukocyte migration inhibition in a Teflon skin chamber with and without addition of antigen, which approaches to testing under in vivo conditions. In 10 of 11 tuberculin allergics, 6 of 7 chromate allergics, and 7 penicillin allergics, a clear inhibition of leucocyte migration after addition of antigen could be observed (maximum 16 h after addition of antigen). In 16 unsensitised controls no significant migration inhibition appeared. Possible advantages of this method over in vitro methods and intracutaneous tests are discussed.