Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

AMPA and NMDA receptor antagonists do not decrease hippocampal glutamate concentrations during transient global

M Matsumoto1, M H Zornow, M S Scheller

  • 1Department of Anesthesiology, University of California, San Diego, La Jolla 92093-0629.

Anesthesiology
|October 1, 1992
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Xenon anesthesia for all, or only a select few?

Anaesthesia·2016
Same author

Concentrations of extracellular free zinc (pZn)e in the central nervous system during simple anesthetization, ischemia and reperfusion.

Experimental neurology·2006
Same author

The impact of longer-than-average anesthesia times on the billing of academic anesthesiology departments.

Anesthesia and analgesia·2001
Same author

Designing meaningful industry metrics for clinical productivity for anesthesiology departments.

Anesthesia and analgesia·2001
Same author

Lubeluzole inhibits accumulation of extracellular glutamate in the hippocampus during transient global cerebral ischemia.

Brain research·2001
Same author

The combination of lamotrigine and mild hypothermia prevents ischemia-induced increase in hippocampal glutamate.

Journal of neurosurgical anesthesiology·2001

This study investigated glutamate feedback loops during brain ischemia. Selective AMPA or NMDA receptor antagonists did not alter excitatory amino acid levels, suggesting no positive feedback loop exists in the hippocampus.

Area of Science:

  • Neuroscience
  • Neuropharmacology
  • Cerebrovascular Physiology

Background:

  • Elevated extracellular glutamate during cerebral ischemia may involve a positive glutamatergic feedback loop.
  • Understanding this mechanism is crucial for developing neuroprotective strategies.

Purpose of the Study:

  • To investigate the role of AMPA and NMDA receptors in hippocampal glutamate release during transient global ischemia and reperfusion.
  • To determine if a positive glutamatergic feedback loop exists via AMPA or NMDA autoreceptors.

Main Methods:

  • Global cerebral ischemia was induced in rabbits using neck tourniquet and systemic hypotension.
  • In vivo microdialysis monitored hippocampal extracellular concentrations of glutamate, aspartate, and glycine.
  • Animals were treated with NBQX (AMPA antagonist), MK801 (NMDA antagonist), or dextrose prior to ischemia.

Related Experiment Videos

Main Results:

  • Peak concentrations of glutamate, aspartate, and glycine increased significantly during reperfusion compared to pre-ischemic levels.
  • No significant differences in amino acid concentrations were observed between the NBQX, MK801, and dextrose groups.
  • This indicates that AMPA or NMDA receptor antagonism did not affect extracellular excitatory amino acid levels during ischemia/reperfusion.

Conclusions:

  • The findings do not support a positive feedback loop for glutamate mediated by AMPA or NMDA autoreceptors in the hippocampus during transient global ischemia.
  • This research challenges existing hypotheses regarding glutamate regulation during ischemic events.