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Detection of bovine alpha s1-casein genomic variants using the allele-specific polymerase chain reaction
1BioMolecular Research Department, W. R. Grace & Co.-Conn., Columbia, Maryland 21044.
Animal Genetics
|January 1, 1992
Abstract:
A method was developed to distinguish between genotypic variants B and C of bovine alpha s1-casein, using the allele-specific polymerase chain reaction (ASPCR). The alpha s1-casein genotype determined for 17 Jersey cows by the ASPCR method was confirmed by typing the alpha s1-casein milk proteins on isoelectric focusing gels. Using the ASPCR method described, rapid analysis of the alpha s1-casein genotype of bulls is now possible. In addition, kappa-casein genotypes can be determined from the same PCR reaction.