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Purification of testicular transglutaminase by hydrophobic chromatography on phenyl-sepharose
1Istituto di Chimica Biologica, Università di Ferrara, Italy.
Biochemistry International
|August 1, 1992
Abstract:
We developed a procedure for purification to homogeneity of cytosolic transglutaminase from bovine testes, by means of anion exchange, size exclusion and hydrophobic chromatography. The crucial step is chromatography on Phenyl sepharose, with specific elution by the inhibitor GTP. The enzyme displays the properties of type 2 transglutaminases.
