Characterization of chicken intestinal brush border membrane Na/H exchange

1. Na/H exchange is the major pathway for Na uptake in brush border membrane vesicles from chicken small intestine. Hanes-Woolf analysis demonstrated that Na and H competed at the same extravesicular site. The KNa for Na+ at extravesicular pH 6.6 is 35 mM and at pH 7.4, 12 mM. 2. Similar to mammalian intestinal cells, the Na/H exchanger does not appear to have an internal proton modifier site. Varying intravesicular pH from 6.1 to 7.8 stimulates uptake, but a sigmoidal relationship is not observed. 3. The ability of several amiloride analogs to inhibit the exchanger was tested and the inhibitory profile was similar, but not identical to Na/H exchangers in mammalian tissues. The potency series (from most to least potent) is hexamethylamiloride approximately ethylisopropylamiloride > methylisobutylamiloride > dimethylamiloride >> amiloride.