Related Experiment Videos
Mapping Creb-1 to chromosome 1 in the mouse
C H Barton1, J W Ajioka, T I Roach
1University of Cambridge Clinical School, Department of Medicine, Addenbrooke's Hospital, Cambridge, United Kingdom.
Genomics
|November 1, 1992
Summary
This study maps the Creb-1 gene on mouse chromosome 1 using genetic markers. Creb-1 is located approximately 1 cM distal to Cryg and 7 cM proximal to Lsh/Vil.
Area of Science:
- Genetics
- Genomics
- Molecular Biology
Background:
- The CREB1 gene, encoding the cyclic AMP response element DNA binding protein, was previously mapped to human chromosome 2.
- Gene mapping is crucial for understanding genetic diseases and functions.
Purpose of the Study:
- To precisely map the Creb-1 gene on mouse chromosome 1.
- To determine the genetic linkage of Creb-1 with the Cryg and Lsh/Vil loci.
Main Methods:
- Utilized a panel of 207 backcross mice for genetic mapping.
- Employed reverse-transcribed, polymerase chain reaction-amplified cDNA probes for Creb-1.
- Analyzed restriction fragment length polymorphisms (RFLPs) using BglII and DraI endonucleases.
Main Results:
- Identified RFLPs for Creb-1 in parental mouse strains.
- Scored recombinants between Creb-1, Cryg, and Lsh/Vil in a subpanel of mice.
- Established the genetic order and distances: Cryg - 1 cM - Creb-1 - 7 cM - Lsh/Vil.
Conclusions:
- The Creb-1 gene is localized to mouse chromosome 1.
- Provides precise mapping data for Creb-1 relative to established genetic markers.