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Immunosuppressive factor from liver and its influence on T cell development
D Kazansky1, N Nastoyashchaya, M Lomakin
1Institute of Immunology, Moscow, Russia.
An endogenous immunosuppressive factor from liver, named ISF-70, of 70 kDa was studied with respect to mouse thymocyte maturation and expression of differentiation antigens in vitro. This factor induced dose-dependent alternative changes in thymocyte viability curves for 72 h incubation. Augmentation of viable cells was accompanied by a decrease in DNA synthesis and an increase in Lyt-2-positive cells. A correlation was observed between thymocyte proliferation, Lyt-2 expression and the action of ISF-70. It was found that ISF-70 distribution on thymocytes coincided with that of Lyt-2 antigen. The role of this factor in thymocyte developmental pathways and self-tolerance is discussed.
An endogenous immunosuppressive factor from liver, named ISF-70, of 70 kDa was studied with respect to mouse thymocyte maturation and expression of differentiation antigens in vitro. This factor induced dose-dependent alternative changes in thymocyte viability curves for 72 h incubation. Augmentation of viable cells was accompanied by a decrease in DNA synthesis and an increase in Lyt-2-positive cells. A correlation was observed between thymocyte proliferation, Lyt-2 expression and the action of ISF-70. It was found that ISF-70 distribution on thymocytes coincided with that of Lyt-2 antigen. The role of this factor in thymocyte developmental pathways and self-tolerance is discussed.