Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunosuppressive factor from liver and its influence on T cell development.

D Kazansky1, N Nastoyashchaya, M Lomakin

  • 1Institute of Immunology, Moscow, Russia.

Immunology Letters
|June 1, 1992
PubMed
Summary

A liver-derived immunosuppressive factor (ISF-70) impacts mouse thymocyte maturation. It promotes viable cell increase and Lyt-2 antigen expression, suggesting a role in immune development and self-tolerance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diverse repertoire of the MHC class II-peptide complexes is required for presentation of viral superantigens.

Journal of immunology (Baltimore, Md. : 1950)ยท2001
See all related articles

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • The liver produces endogenous immunosuppressive factors.
  • Thymocyte maturation is crucial for T cell development and immune tolerance.
  • Understanding factors influencing thymocyte differentiation is key to immune regulation.

Purpose of the Study:

  • To investigate the effects of a novel liver-derived immunosuppressive factor (ISF-70) on mouse thymocyte maturation in vitro.
  • To analyze the impact of ISF-70 on thymocyte viability, DNA synthesis, and differentiation antigen expression.
  • To explore the correlation between ISF-70 activity, thymocyte proliferation, and Lyt-2 antigen expression.

Main Methods:

  • In vitro study of mouse thymocytes incubated with ISF-70.
  • Analysis of thymocyte viability curves over 72 hours.

Related Experiment Videos

  • Measurement of DNA synthesis and expression of differentiation antigens (Lyt-2).
  • Immunofluorescence to determine ISF-70 distribution on thymocytes.

    • Main Results:

    • ISF-70 induced dose-dependent changes in thymocyte viability.
    • Increased viable thymocytes were observed with decreased DNA synthesis.
    • A significant increase in Lyt-2-positive cells was noted.
    • ISF-70 distribution on thymocytes correlated with Lyt-2 antigen localization.

    Conclusions:

    • ISF-70, a 70 kDa liver factor, modulates mouse thymocyte maturation.
    • The factor influences thymocyte proliferation and promotes the expression of the Lyt-2 differentiation antigen.
    • ISF-70 may play a role in thymocyte developmental pathways and the maintenance of self-tolerance.