Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Plasmid-pattern analysis in the differentiation of Staphylococcus epidermidis isolates.

C M Ferreirós1, M J Souto, M T Criado

  • 1Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Santiago de Compostela, Spain.

The Journal of Hospital Infection
|September 1, 1992
PubMed
Summary

Plasmid patterns in Staphylococcus epidermidis did not distinguish between invasive and carrier strains. However, plasmid analysis proved useful for tracking the spread of these bacteria in epidemiological investigations.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro evaluation of the antibacterial and osteogenic activity promoted by selenium-doped calcium phosphate coatings.

Biomedical materials (Bristol, England)·2017
Same author

Induction of immune responses by purified outer membrane protein complexes from Neisseria meningitidis.

Vaccine·2011
Same author

Reacetylated chitosan microspheres for controlled delivery of anti-microbial agents to the gastric mucosa.

Journal of microencapsulation·2003
Same author

[Influence of adjuvants on the ability of anti-Tbp antibodies to block transferrin binding, iron uptake and growth of Neisseria meningitis].

Enfermedades infecciosas y microbiologia clinica·2002
Same author

Analysis of Neisseria lactamica antigens putatively implicated in acquisition of natural immunity to Neisseria meningitidis.

FEMS immunology and medical microbiology·2002
Same author

In vitro induction of memory-driven responses against Neisseria meningitidis by priming with Neisseria lactamica.

Vaccine·2002

Area of Science:

  • Microbiology
  • Molecular Biology
  • Epidemiology

Background:

  • Staphylococcus epidermidis is a common bacterium.
  • Distinguishing between invasive and carrier strains is important for clinical and epidemiological purposes.
  • Plasmids are known to play a role in bacterial virulence and adaptation.

Purpose of the Study:

  • To investigate the association between plasmid profiles and the origin (invasive vs. carrier) of Staphylococcus epidermidis isolates.
  • To determine if plasmid pattern analysis can differentiate between these two types of isolates.

Main Methods:

  • Agarose gel electrophoresis was used to analyze plasmids.
  • Twenty-nine Staphylococcus epidermidis isolates from invasive and carrier origins were studied.

Related Experiment Videos

Main Results:

  • Plasmid patterns were unique to individual strains.
  • No specific plasmid size or pattern correlated with either invasive or carrier isolates.
  • Plasmid analysis did not differentiate between the two isolate categories.

Conclusions:

  • Plasmid profiling alone cannot distinguish invasive from carrier Staphylococcus epidermidis strains.
  • Plasmid pattern analysis is a valuable tool for epidemiological investigations of Staphylococcus epidermidis.