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Related Experiment Videos

Prolactin gene expression in human thymocytes.

K D O'Neal1, D W Montgomery, T M Truong

  • 1Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.

Molecular and Cellular Endocrinology
|September 1, 1992
PubMed
Summary
This summary is machine-generated.

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Prolactin production by immune cells.

Lupus·2001

Lymphocytes produce prolactin (PRL), a hormone typically found in the pituitary gland. This study confirms thymocytes express PRL, suggesting it acts as a T cell-derived cytokine.

Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Recent evidence suggests lymphocytes produce prolactin (PRL).
  • The role of PRL beyond the pituitary gland is under investigation.
  • Understanding non-pituitary sources of PRL is crucial for immunology and endocrinology.

Purpose of the Study:

  • To clone and characterize prolactin (PRL) expressed in human thymocytes.
  • To investigate the presence and regulation of PRL mRNA in non-pituitary cell lines.
  • To determine if PRL functions as a T cell-derived cytokine.

Main Methods:

  • cDNA cloning and sequence analysis of thymocyte PRL.
  • RNA blot analysis to compare PRL mRNA size from thymocytes and pituitary.
  • Detection of PRL mRNA in human cell lines (Jurkat T, HeLa, JEG).

Related Experiment Videos

  • Glucocorticoid treatment to assess inhibition of PRL gene expression in JEG cells.

    • Main Results:

    • Human thymocytes express a 23 kDa PRL protein identical to pituitary PRL.
    • Thymocyte PRL mRNA is approximately 170 nucleotides larger than pituitary PRL mRNA.
    • PRL mRNA is detectable in Jurkat T, HeLa, and JEG cell lines.
    • Glucocorticoids inhibit PRL gene expression in JEG cells.

    Conclusions:

    • Prolactin (PRL) is produced by human thymocytes.
    • PRL gene expression is present in various non-pituitary human cell lines.
    • PRL may function as a T cell-derived cytokine, with its expression potentially regulated by glucocorticoids.