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Related Experiment Videos

Regulated Ca2+ signalling through leukocyte CD11b/CD18 integrin.

D C Altieri1, S J Stamnes, C G Gahmberg

  • 1Department of Immunology, Scripps Research Institute, La Jolla, CA 92037.

The Biochemical Journal
|December 1, 1992
PubMed
Summary

Leukocyte integrin CD18 cross-linking triggers a calcium (Ca2+) signaling response in immune cells, modulated by cell differentiation and CD11b association. This calcium signaling is crucial for immune cell function.

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Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Signaling

Background:

  • Leukocyte integrins, such as CD11/CD18, are key regulators of immune cell adhesion.
  • The role of these differentiation molecules in early transmembrane signaling events remains incompletely understood.

Purpose of the Study:

  • To investigate the participation of leukocyte integrin CD18 in early transmembrane signaling.
  • To elucidate the mechanisms of CD18-mediated calcium (Ca2+) signaling in immune cells.

Main Methods:

  • Monoclonal antibody (mAb) cross-linking of CD18 on THP-1 cells and peripheral blood monocytes.
  • Digitalized imaging of cytosolic free Ca2+ concentration ([Ca2+]i) in single adherent cells.
  • Analysis of Ca2+ signaling during T-cell activation and CD11b/CD18 avidity for fibrinogen.

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Main Results:

  • Cross-linking CD18 increased [Ca2+]i in THP-1 cells and a subset of peripheral blood monocytes, with the response modulated by differentiation.
  • CD18 cross-linking induced Ca2+ signaling in a subset of resting T lymphocytes, abrogated upon activation.
  • Occupancy of CD11b, but not CD11a or CD11c, increased [Ca2+]i in monocytic cells, coupled with increased CD11b/CD18 avidity.

Conclusions:

  • CD18 occupancy transduces a stimulatory Ca2+ signal critical for immune cell function.
  • This signaling is regulated by cell activation/differentiation status and association with the CD11b alpha-subunit.
  • These intrinsic signaling properties may regulate the ligand recognition of leukocyte integrins.