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Updated: Aug 10, 2026
10:31
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Immunophenotypical characterization of inflammatory cellular infiltrates in tinea
Acta Dermato-Venereologica
|September 1, 1992
Summary
Acute tinea infections involve specific immune cells like T helper lymphocytes and macrophages, with few B cells. This study highlights the role of Langerhans cells and macrophages in the skin
Area of Science:
- Dermatology
- Immunology
- Mycology
Background:
- The pathogenesis of acute tinea infections remains incompletely understood.
- Inflammatory cellular infiltrates play a key role in skin infections.
Purpose of the Study:
- To analyze the inflammatory cellular infiltrates in acute tinea.
- To elucidate the pathogenetic pathways of acute tinea.
Main Methods:
- Lesional punch biopsies were obtained from patients with acute tinea.
- Immunophenotypization of inflammatory cells was performed using monoclonal antibodies.
- Quantification of T cells, B cells, macrophages, and activation markers in dermal and epidermal layers.
Main Results:
- Dermal infiltrates were predominantly T helper lymphocytes (memory type), macrophages, and Langerhans cells, with minimal B cells.
- A high proportion of inflammatory cells expressed activation markers.
- Epidermal accumulations of Langerhans cells and LeuM5+ dendritic macrophages were observed near fungal elements.
Conclusions:
- Acute tinea exhibits a distinct inflammatory infiltrate, primarily composed of T helper lymphocytes and macrophages.
- The findings suggest a potential role for Langerhans cells and LeuM5+ macrophages in acute tinea pathogenesis.
- Acute tinea may serve as a model for studying the functional interplay between Langerhans cells and macrophages in dermatoses.
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