Ethanol radical-induced protein-DNA crosslinking. A radiolysis study

H Schuessler¹, G Schmerler-Dremel, J Danzer

¹Institut für Radiologie der Universität Erlangen-Nürnberg, Germany.

International Journal of Radiation Biology

|November 1, 1992

Abstract:

Aqueous solutions of double-stranded DNA from calf thymus and bovine serum albumin (BSA) were irradiated at pH 7 under N2O and N2 in the presence of 10(-1) mol dm-3 ethanol, which was partly 14C-labelled. Ethanol protects DNA from strand breakage by scavenging OH radicals, but ethanol radicals induce protein-DNA crosslinks. Ethanol radicals react readily with BSA mainly by addition. They react also with double-stranded DNA, but produce crosslinking only very slowly. Based on these results the following mechanism is proposed: ethanol radicals bind to BSA producing protein radicals which become crosslinked to DNA.

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