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Hyperglycemic athymic nude mice: factors affecting in vitro insulin secretion
A Zeidler1, P Edwards, J Goldman
1Diabetes, Hypertension, and Nutrition, University of Southern California School of Medicine, Los Angeles 90033.
The American Journal of Physiology
|December 1, 1992
Summary
Athymic nude male (ANM) mice develop hyperglycemia and low insulin levels. Their isolated pancreases show normal glucose-stimulated insulin secretion, suggesting other factors cause hypoinsulinemia in vivo.
Area of Science:
- Endocrinology
- Diabetology
- Animal Models
Background:
- Athymic nude male (ANM) mice from the University of Southern California (USC) spontaneously develop hyperglycemia and relative hypoinsulinemia.
- Understanding the mechanisms behind this non-insulin-dependent diabetes mellitus model is crucial.
Purpose of the Study:
- Investigate factors influencing insulin secretion in ANM-USC mice.
- Compare in vitro glucose-induced insulin secretion between ANM-USC and control mice.
- Assess somatostatin's inhibitory effect and secretion variability.
Main Methods:
- Isolated perfused mouse pancreas technique.
- Comparison of glucose-induced insulin secretion in ANM-USC and BALB/c control mice.
- Evaluation of somatostatin inhibition and long-term secretion variability.
Main Results:
- Glucose-induced insulin secretion was biphasic in both ANM-USC and control mice.
- Insulin secretion from ANM-USC pancreases was quantitatively similar to or greater than controls.
- ANM-USC pancreases showed relative resistance to somatostatin inhibition compared to controls.
- Insulin secretion variability was higher in control mice over the study period.
Conclusions:
- Hyperglycemic ANM-USC mice do not exhibit diminished in vitro insulin secretion.
- Relative hypoinsulinemia in vivo suggests circulating factors, potentially excluding somatostatin, contribute to the insulinopenic state.
- This model offers insights into non-insulin-dependent diabetes mellitus pathogenesis.