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Related Experiment Videos

Le(X) and related structures as adhesion molecules.

S Hakomori1

  • 1Biomembrane Institute, Seattle, WA 98119.

The Histochemical Journal
|November 1, 1992
PubMed
Summary
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Le(x) (alpha 1-->3 fucosylated type 2 chain) mediates cell adhesion and aggregation through Ca(2+)-dependent homotypic binding. Sialylated derivatives like SLe(x) and SLe(a) are key targets for selectin-mediated cell adhesion.

Area of Science:

  • Glycobiology
  • Cell Adhesion Biology
  • Developmental Biology

Background:

  • Le(x) (alpha 1-->3 fucosylated type 2 chain) is an adhesion molecule involved in Ca(2+)-mediated homotypic binding.
  • High surface expression of Le(x) leads to strong cell self-aggregation via Le(x)-Le(x) interactions.
  • This interaction plays a role in embryo compaction and F9 teratocarcinoma cell autoaggregation.

Purpose of the Study:

  • To review data supporting the concept of Le(x)-Le(x) mediated cell adhesion and aggregation.
  • To discuss the role of Le(x)-Le(x) interactions in specific biological processes.
  • To explore the function of sialylated derivatives (SLe(x), SLe(a)) in selectin-dependent adhesion.

Main Methods:

  • Review of existing scientific literature and data.

Related Experiment Videos

  • Analysis of cell aggregation assays and adhesion studies.
  • Examination of molecular structures and functional roles of carbohydrate epitopes.

    • Main Results:

    • Le(x)-Le(x) interactions drive homotypic cell binding and aggregation, potentially reinforced by other adhesion systems.
    • Sialyl Lewis x (SLe(x)) and its isomer Sialyl Lewis a (SLe(a)) are critical for selectin-mediated adhesion.
    • These sialylated epitopes are primarily found on O-linked glycoproteins, suggesting O-glycosylation inhibition as a therapeutic target.

    Conclusions:

    • Le(x) and its derivatives are crucial for various cell adhesion processes, including early development and immune cell trafficking.
    • Selectin-ligand interactions involving SLe(x) and SLe(a) are initiated by these epitopes on O-glycosylated proteins.
    • Understanding these adhesive properties is vital, especially given Le(x) abundance in the central nervous system and its changing expression patterns.