Related Experiment Video
Updated: Jul 27, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
[Vibrio cholerae toxin B subunit gene expressed in a Salmonella vaccine strain]
Abstract:
This paper reports that the V. cholerae toxin B subunit (ctx B) gene was inserted into pYA 248 plasmid with the aspartate beta-semialdehyde dehydrogenase (asd)gene and the recombinant plasmid was transformed into S. typhimurium deleting asd gene. Results showed that ctx B gene was highly expressed and secreted into midium. This strain was able to colonize in the intestinal epithelium. Oral immunity and general immunity could produce antibodies at high level and enhance cellular immune responses. The animals orally inoculated with S. typhimurium x 4072 (pYA-ctx B) vaccine had remarkable protection against virulent V. cholear 569B strain and S. typhimurium strain. Use of such system provides useful method for oral vaccine.
Related Concept Videos
Stringent Response in E. coli
Vaccine Production
Bacterial Toxins
Regulation of Bacterial Virulence
Bacterial Gastroenteritis
Cholera

