Related Experiment Videos
Expression of p53 gene in B-CLL peripheral lymphocytes
D Gandini1, S Moretti, A Latorraca
1Istituto di Chimica Biologica, Centro di Studi Biochimici delle Patologie del Genoma Umano.
Bollettino Della Societa Italiana Di Biologia Sperimentale
|October 1, 1992
Abstract:
The p53 tumor suppressor gene expression has been studied in 23 B-CLL cases at different clinical stages. The analysis failed to show a direct correlation with each stage, but the significantly lower frequency of a BglII RFLP in the pathologic population suggests a role of this gene in B-CLL. Northern Blot analysis showed the expression of p53 mRNA in all the B-CLL cases. A protocol for the RT-PCR methods was set up to study a very small amount of materials which should be better used for sequence analysis.