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Related Experiment Videos

Isolation and characterization of a cDNA encoding a synaptonemal complex protein.

Q Chen1, R E Pearlman, P B Moens

  • 1Department of Biology, York University, Downsview, Ont., Canada.

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
|October 1, 1992
PubMed
Summary

Researchers identified the SC65 gene, crucial for the rat synaptonemal complex. This gene product is located on the synaptonemal complex and shows varied transcription levels across rat tissues.

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The genomic structure of SYCP3, a meiosis-specific gene encoding a protein of the chromosome core.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • The synaptonemal complex is essential for homologous chromosome pairing during meiosis.
  • Understanding the molecular components of the synaptonemal complex is key to deciphering meiotic processes.

Purpose of the Study:

  • To clone and characterize the gene encoding the 65-kilodalton antigen (SC65) of the rat synaptonemal complex.
  • To determine the localization, expression pattern, and potential functional motifs of the SC65 protein.

Main Methods:

  • Cloning of the SC65 gene using lambda gt11 and lambda ZAPII cDNA expression libraries.
  • Screening with polyclonal antibodies against rat synaptonemal complex proteins.
  • Immunological analysis and DNA/protein sequence analysis.

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Main Results:

  • The SC65 gene was cloned, encoding a 431-amino acid protein (50,000 Da).
  • The SC65 protein localizes to the synaptonemal complex between homologous chromosome cores.
  • SC65 gene transcription is detected in testis, brain, and heart, with lower levels in the liver.

Conclusions:

  • The SC65 gene product is a component of the rat synaptonemal complex.
  • The protein contains potentially functional motifs like acidic regions and phosphorylation sites.
  • SC65 shows no significant similarity to known proteins, suggesting a novel function in meiosis or other cellular processes.